All major employers listed are located in Harrison County.
| Name | City | Est. # Employees | Business Type |
|---|---|---|---|
| Keesler Air Force Base | Biloxi | 12222 | Military |
| Naval Construction Battalion Center | Gulfport | 5400 | Military |
| Memorial Hospital | Gulfport | 3290 | Health Care |
| Beau Rivage Resort & Casino | Biloxi | 2366 | Gaming |
| VA Gulf Coast Veterans Health Care System | Biloxi | 1985 | Health Care |
| Harrison County School District | Gulfport | 1721 | Education |
| IP Casino Resort Spa | Biloxi | 1656 | Gaming |
| Island View Casino Resort | Gulfport | 1009 | Gaming |
| DuPont DeLisle | Pass Christian | 888 | Manufacturing |
| Hancock Bank | Gulfport | 864 | Financial |
| Gulfport School District | Gulfport | 817 | Education |
| Hard Rock Hotel & Casino | Biloxi | 772 | Gaming |
| Biloxi Regional Medical Center | Biloxi | 750 | Health Care |
| Ingalls Shipbuilding, a division of Huntington Ingalls | Gulfport | 730 | Manufacturing |
| Mississippi Power Company | Gulfport | 728 | Utilities |
| Grand Biloxi Casino Hotel Spa | Biloxi | 715 | Gaming |
| Biloxi Public School District | Biloxi | 665 | Education |
| Palace Casino Resort | Biloxi | 630 | Gaming |
| City of Biloxi | Biloxi | 623 | Government |
| Isle Casino Hotel | Biloxi | 605 | Gaming |
| City of Gulfport | Gulfport | 605 | Government |
| Walmart Supercenter | Gulfport | 585 | Retail/Wholesale |
| Boomtown Casino Biloxi | Biloxi | 556 | Gaming |
| Walmart Supercenter | DIberville | 550 | Retail/Wholesale |
| Treasure Bay Casino and Hotel | Biloxi | 549 | Gaming |
| Garden Park Medical Center | Gulfport | 525 | Health Care |
| Gulf Ship, LLC | Gulfport | 512 | Manufacturing |
| Prime Outlets | Gulfport | 450 | Retail/Wholesale |
| Trinity Yachts | Gulfport | 400 | Manufacturing |
| Long Beach School District | Long Beach | 396 | Education |
| Gulf Coast Pre-Stress | Pass Christian | 360 | Manufacturing |
| Roy Anderson Corp. | Gulfport | 336 | Construction |
| RPM Pizza, LLC | Gulfport | 322 | Food Services |
| Global Financial Aid Services | Gulfport | 300 | Services |
| Mississippi Gulf Coast Community College | Gulfport | 273 | Education |
| Pass Christian School District | Pass Christian | 259 | Education |
| W. G. Yates & Sons Construction | Biloxi | 250 | Construction |
| Walmart | Biloxi | 250 | Retail |
| Sun Herald Multi Media | Gulfport | 230 | Information |
| Triton Systems, Inc. | Long Beach | 207 | Manufacturing |
| United States Marine, Inc. | Gulfport | 200 | Manufacturing |
| Rex Distributing Company, Inc. | Gulfport | 116 | Retail/Wholesale |
| F.E.B. Distributing Company, Inc. | Gulfport | 112 | Retail/Wholesale |
| Gulf Coast Laundry | Gulfport | 110 | Services |
| Coca-Cola Bottling Co. | Gulfport | 100 | Wholesale |
| Geri Care | Gulfport | 85 | Manufacturing |
| Goldin Metals, Inc. | Gulfport | 70 | Manufacturing |
| Bay Technical Associates, Inc | Long Beach | 68 | Manufacturing |
| Hartson-Kennedy Cabinet Top Co., Inc. | Gulfport | 60 | Manufacturing |
| Gulfport-Biloxi International Airport | Gulfport | 53 | Transportation |
| Golden Gulf Coast Packing Co., Inc. | Biloxi | 50 | Seafood Processing |
| Geo Pave | Gulfport | 45 | Construction |
| Speciality Machine Works, Inc. | Gulfport | 45 | Manufacturing |
| Metal Tech, Inc. | Gulfport | 36 | Manufacturing |
| Soprema USA, Inc. | Gulfport | 17 | Manufacturing |
| Specialty Hose & Fabrication, Inc. | Gulfport | 5 | Manufacturing |
