All major employers listed are located in Harrison County.
| Name | City | Est. # Employees | Business Type |
|---|---|---|---|
| Keesler Air Force Base | Biloxi | 12328 | Military |
| Naval Construction Battalion Center | Gulfport | 5800 | Military |
| Memorial Hospital | Gulfport | 2894 | Health Care |
| Beau Rivage Resort & Casino | Biloxi | 2819 | Gaming |
| IP Casino Resort Spa | Biloxi | 1912 | Gaming |
| VA Gulf Coast Veterans Health Care System | Biloxi | 1780 | Health Care |
| Harrison County School District | Gulfport | 1649 | Education |
| Mississippi Power Company | Gulfport | 1253 | Utilities |
| Island View Casino Resort | Gulfport | 1188 | Gaming |
| Hard Rock Hotel & Casino | Biloxi | 923 | Gaming |
| Gulfport School District | Gulfport | 900 | Education |
| Hancock Bank | Gulfport | 864 | Financial |
| Grand Biloxi Casino Hotel Spa | Biloxi | 815 | Gaming |
| Palace Casino Resort | Biloxi | 793 | Gaming |
| Biloxi Public School District | Biloxi | 734 | Education |
| Huntington Ingalls Industries, Inc. | Gulfport | 730 | Manufacturing |
| Isle Casino Hotel | Biloxi | 675 | Gaming |
| City of Biloxi | Biloxi | 660 | Government |
| Biloxi Regional Medical Center | Biloxi | 653 | Health Care |
| City of Gulfport | Gulfport | 605 | Government |
| Boomtown Casino Biloxi | Biloxi | 592 | Gaming |
| Walmart Supercenter | Gulfport | 585 | Retail/Wholesale |
| Treasure Bay Casino and Hotel | Biloxi | 564 | Gaming |
| Garden Park Medical Center | Gulfport | 560 | Health Care |
| Walmart Supercenter | DIberville | 550 | Retail/Wholesale |
| DuPont DeLisle | Pass Christian | 488 | Manufacturing |
| Prime Outlets | Gulfport | 450 | Retail/Wholesale |
| Long Beach School District | Long Beach | 414 | Education |
| Trinity Yachts | Gulfport | 400 | Manufacturing |
| Mississippi Gulf Coast Community College | Gulfport | 398 | Education |
| Gulf Coast Pre-Stress | Pass Christian | 360 | Manufacturing |
| Roy Anderson Corp. | Gulfport | 336 | Construction |
| W. G. Yates & Sons Construction | Biloxi | 332 | Construction |
| RPM Pizza, LLC | Gulfport | 322 | Food Services |
| Global Financial Aid Services | Gulfport | 300 | Services |
| Gulf Ship, LLC | Gulfport | 292 | Manufacturing |
| Pass Christian School District | Pass Christian | 274 | Education |
| Walmart | Biloxi | 250 | Retail |
| Sun Herald Multi Media | Gulfport | 230 | Information |
| United States Marine, Inc. | Gulfport | 200 | Manufacturing |
| Triton Systems, Inc. | Long Beach | 150 | Manufacturing |
| Rex Distributing Company, Inc. | Gulfport | 116 | Retail/Wholesale |
| F.E.B. Distributing Company, Inc. | Gulfport | 112 | Retail/Wholesale |
| Gulf Coast Laundry | Gulfport | 106 | Services |
| Coca-Cola Bottling Co. | Gulfport | 100 | Wholesale |
| Goldin Metals, Inc. | Gulfport | 70 | Manufacturing |
| Bay Technical Associates, Inc | Long Beach | 68 | Manufacturing |
| Hartson-Kennedy Cabinet Top Co., Inc. | Gulfport | 60 | Manufacturing |
| Gulfport-Biloxi International Airport | Gulfport | 53 | Transportation |
| Speciality Machine Works, Inc. | Gulfport | 45 | Manufacturing |
| Metal Tech, Inc. | Gulfport | 36 | Manufacturing |
| Soprema USA, Inc. | Gulfport | 17 | Manufacturing |
| Specialty Hose & Fabrication, Inc. | Gulfport | 5 | Manufacturing |
